PBS Biotech
Cell Therapy · MSCs

Mesenchymal Stem / Stromal Cells

Scalable, xeno-free MSC manufacturing on microcarriers or aggregates, from flasks to 80 L, with extracellular-vesicle potential built in.

0.1–50 L
demonstrated scales
Xeno-free
fed-batch process
↑ EV
secretion vs planar
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Why Vertical-Wheel® for MSCs

MSCs and MSC-derived EVs are in numerous clinical trials, driving demand for billions to trillions of cells per lot. The Vertical-Wheel® low-shear environment protects these shear-sensitive cells while distributing gas and nutrients uniformly.

Gentle on shear-sensitive cells

Uniform low-shear mixing protects hMSC viability and phenotype while keeping microcarriers or aggregates suspended.

Microcarrier-optimized

Even microcarrier suspension, including customizable and dissolvable options that improve harvest and direct visualization.

Fed-batch productivity

Xeno-free fed-batch processes lift final cell yields and EV cargo across 0.1 L to 50 L scales.

Applications

What You Can Build.

Workflows our customers and collaborators run on the Vertical-Wheel® platform, every one backed by published data or a protocol below.

  • BM-MSC expansion
  • Cord-blood MSC
  • Adipose-derived MSC
  • Microcarrier culture
  • Fed-batch processes
  • MSC-derived EVs
0.1–50 L

A xeno-free hMSC process maintained critical quality attributes across 0.1 L, 3 L, 15 L and 50 L Vertical-Wheel® scales.

Source · RoosterBio × PBS Biotech application note, 2025
The Technology

One Wheel. Gentle, Homogeneous Mixing.

Every result on this page comes back to the same mechanism: a single vertically-oriented wheel that suspends cells uniformly at low shear, from 0.1 L to 80 L.

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Ready to Scale Your MSCs Process?

Talk to an application scientist about a Vertical-Wheel® path tuned to your cell type and timeline.