Use of 60 Micron Plastic (PE) Retention Filter for Perfusion MX with PBS-3 Vertical-Wheel® Bioreactor

This application note provides materials, assembly instructions, and operating guidance for using a 60 micron plastic polyethylene retention filter to perform perfusion medium exchange in a PBS-3 Vertical-Wheel bioreactor. The filter is intended for pluripotent stem cell cultures grown as aggregates or on microcarriers. The document specifies recommended tubing, connectors, reservoirs, pump components, dip-tube placement, and construction of the fresh- and spent-medium lines. It also describes sterilization precautions, complete system installation, pump calibration, line priming, medium-exchange initiation, and filter disposal. Recommended flow rates are provided for 0.5, 1, and 2 vessel volumes per day at a 3 L working volume over 24 hours. The protocol notes that the filter is supplied nonirradiated, recommends autoclaving the assembled system before use, and advises against autoclaving the retention filter more than once.