MSC Culture in the PBS-Mini Bioreactor System
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel technology. The document covers the complete workflow from recovery of cryopreserved MSCs and expansion in planar culture through inoculation, suspension culture, sampling, medium exchange, and harvest in three-dimensional bioreactor systems. Recommended process parameters are based on internal optimization studies using multiple MSC lines and media formulations. Guidance is provided for microcarrier preparation, cell attachment, culture monitoring, partial harvest, and full harvest operations. The protocol is intended as a starting framework for users implementing MSC expansion processes and emphasizes adaptation of operating parameters to specific cell lines, media systems, and manufacturing objectives.
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel bioreactors.
This guide provides detailed instructions for setting up, calibrating, and operating the PBS 0.5 Mini Vessel equipped with a 60 µm perfusion filter.
This application note evaluates expansion of human T cells in a PBS Mini Vertical-Wheel bioreactor using CellGenix GMP serum-free, xeno-free…
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