Scalable Manufacturing of Human Mesenchymal Stem/Stromal Cells and Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors

This poster summarizes manufacturing approaches for expansion of both human mesenchymal stem/stromal cells and human induced pluripotent stem cells in Vertical-Wheel bioreactor systems. For MSC processes, the authors present microcarrier-based culture strategies capable of achieving high cell densities with strong harvest recovery across multiple scales. For iPSC cultures, computational fluid dynamics and energy dissipation modeling were applied to establish relationships between agitation conditions and aggregate morphology. These analyses were used to guide scale-up and maintain consistent culture performance between reactor sizes. The work demonstrates how engineering principles can be applied to both adherent and aggregate-based cell manufacturing platforms while supporting reproducible process development and scalable production workflows.