Engineering Human Bone Marrow-Derived Mesenchymal Stromal Cell Aggregates for Enhanced Extracellular Vesicle Secretion in a Vertical-Wheel Bioreactor
This publication investigates large-scale production of extracellular vesicles from three-dimensional aggregates of human bone marrow-derived mesenchymal stromal cells cultured in Vertical-Wheel bioreactors. The authors evaluated the influence of culture media and agitation conditions on metabolic activity, EV biogenesis, EV yield, and molecular cargo composition. Dynamic aggregate culture increased expression of EV biogenesis pathways and enhanced vesicle production relative to static culture systems. Characterization studies confirmed expected EV markers and identified microRNA profiles associated with regenerative and immunomodulatory functions. Functional analyses further demonstrated the ability of the resulting vesicles to influence inflammatory responses in Schwann cell models. The work highlights a scalable bioprocess approach for manufacturing therapeutically relevant MSC-derived extracellular vesicles with improved yield and functional potential.
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel bioreactors.
This protocol provides detailed instructions for expanding mesenchymal stem cells on microcarriers using PBS-Mini bioreactors with Vertical-Wheel…
This guide provides detailed instructions for setting up, calibrating, and operating the PBS 0.5 Mini Vessel equipped with a 60 µm perfusion filter.
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