Defined Biomatrices Accelerate MSC Manufacturing Across Scales

This white paper describes a small-scale manufacturing workflow for human bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs), from isolation and monolayer expansion through microcarrier-based bioreactor culture. MSCs were isolated from cryopreserved bone marrow mononuclear cells using isoMATRIX, expanded in two-dimensional culture on myMATRIX MSC, and transferred to beadMATRIX microcarriers in a PBS-0.1 Mini Vertical-Wheel bioreactor. The study compares these defined biomatrices with competitor products and reports improved isolation efficiency with isoMATRIX, efficient cell expansion across five monolayer passages, and comparable MSC proliferation on beadMATRIX and competitor microcarriers. It presents an integrated, serum- and xeno-free-compatible approach for connecting MSC isolation, seed-train expansion, and three-dimensional bioreactor expansion while maintaining high cell viability and characteristic fibroblast-like morphology.