Optimizing Closed-System iPSC Processes Using a Scaled-Down, Computer-Controlled Parallel Bioreactor Platform

This poster evaluates a computer-controlled parallel Vertical-Wheel bioreactor platform as a scale-down model for human induced pluripotent stem cell process development. Studies assessed dissolved oxygen control, headspace gassing, perfusion media exchange, direct thaw of high-density cell banks, aggregate morphology, growth, metabolism, pluripotency, and closed-system operation. Controlled oxygen addition reduced oxygen limitations encountered in incubator-operated vessels and supported increased cell yield. Directly thawed iPSCs generated consistent aggregates and maintained OCT4 and SOX2 expression under multiple media conditions. Comparisons between the scaled-down system and a 3 L bioreactor showed similar growth rates, aggregate-size distributions, and metabolic profiles when agitation was selected using energy dissipation correlations. The poster also presents integrated retention filters, automated pumping, and closed fluid-handling workflows designed to improve representative process development.